Affiliation:
1. Department of Microbiology, Yale University, New Haven, Connecticut
Abstract
Schwartz, Norman M.
(Yale University, New Haven, Conn.). Suppression of a
lac o°
mutation in
Escherichia coli
. J. Bacteriol.
88:
996–1001. 1964.—A class of
pro
–
markers which can be cotransduced with
lac
map close to the
i
side of the
lac
region. The relative order of markers transferred by Hfr H is
thr leu proA proB lac proC ade
. Nine of ten slowgrowing lactose-utilizing revertants of a
lac o°
mutant are suppressed mutants. The time of entry of
su-lac o-5
from an Hfr H derivative is 27 min.
Su-lac o-5
is separated from the
lac
region by a 10-min interval.
Su-lac o-5
is unstable, restores 7% of the wild-type level of β-galactosidase activity, suppresses a
try
–
mutation, and has a deleterious effect upon growth rate. The primary
lac o°
mutation is interpreted as being a
z
–
polarity mutation which constitutes nonsense.
Su-lac o-5
could act at the level of messenger ribonucleic acid translation converting nonsense to sense by promoting mistakes in protein synthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
22 articles.
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