Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli-Reference-Cited by-同舟云学术

Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli

Published:2015-09-18 Issue:1 Volume:11 Page:80-90
ISSN:1860-6768
Container-title:Biotechnology Journal
language:en
Short-container-title:Biotechnology Journal

Author:

Thakker Chandresh¹²,Lin Kevin¹,Martini‐Stoica Heidi¹³,Bennett George N.¹

Affiliation:

1. Department of BioSciences, Rice University Houston TX USA

2. TOTAL New Energies USA, Inc. Emeryville CA USA

3. Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine Houston TX USA

Funder

National Science Foundation

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201500205

Reference43 articles.

1. High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage

2. A method for generating precise gene deletions and insertions in Escherichia coli

3. Recombineering: a homologous recombination-based method of genetic engineering

4. Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination

5. Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

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