DIHYDROSTREPTOMYCIN, VITAMIN K 2 -COUPLED TETRAZOLIUM REDUCTION, AND OXIDATIVE PHOSPHORYLATION IN ESCHERICHIA COLI

Abstract

Bragg, P. D. (University of British Columbia, Vancouver, B.C., Canada). Dihydrostreptomycin, vitamin K 2 -coupled tetrazolium reduction, and oxidative phosphorylation in Escherichia coli . J. Bacteriol. 88: 1019–1023. 1964.—Dihydrostreptomycin inhibited the vitamin K 2 -coupled pyruvate-triphenyltetrazolium (TTC) reductase system in extracts from antibiotic-sensitive cells, but stimulated this reaction with extracts from a dependent mutant. The effects of nucleotides, phosphate, and uncoupling agents on the vitamin K 2 -TTC system are consistent with the linkage of reduction of the dye through intermediates of oxidative phosphorylation.