Affiliation:
1. Department of Biochemistry, University of British Columbia, Vancouver, British Columbia, Canada
Abstract
Bragg
, P. D. (University of British Columbia, Vancouver, British Columbia, Canada)
and W. J. Polglase
. Effect of dihydrostreptomycin on tetrazolium dye reduction in
Escherichia coli
. J. Bacteriol.
85:
795–800. 1963.—Sonic-disrupted extracts of
Escherichia coli
, grown without added antibiotic (sensitive and resistant), contained (in supernatant of fraction centrifuged at 100,000 ×
g
) a dihydrostreptomycin-inhibitable, succinate-triphenyltetrazolium chloride (TTC) reductase activity. The succinate-TTC reductase activities of extracts of
E. coli
grown in the presence of dihydrostreptomycin (resistant and dependent) were relatively low and were not inhibited by the antibiotic. At a moderate magnesium concentration, the degree of inhibition by dihydrostreptomycin of succinate-TTC reductase activity was sufficiently marked to indicate an important site of action of the antibiotic. Magnesium, putrescine, and spermidine antagonized the action of dihydrostreptomycin in the succinate-TTC reductase system.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
5 articles.
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