Affiliation:
1. Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
Abstract
Benziman, Moshe
(The Hebrew University of Jerusalem, Jerusalem, Israel),
and Y. Galanter
. Flavine adenine dinucleotide-linked malic dehydrogenase from
Acetobacter xylinum
. J. Bacteriol.
88:
1010–1018. 1964.—The properties of the pyridine nucleotide-nonlinked malic dehydrogenase of
Acetobacter xylinum
were investigated in the supernatant fluid obtained by high-speed centrifugation of sonic extracts. Ferricyanide, phenazine methosulfate, and to a lesser extent dichlorophenolindophenol were active as oxidants for malate oxidation. After acid ammonium sulfate precipitation, the enzyme lost its malate-oxidizing activity. The enzyme was reactivated by low concentrations of flavine adenine dinucleotide (FAD) but not by flavine mononucleotide (FMN) or riboflavine. Atabrine inhibited the enzyme, and the inhibition was relieved by FAD but not by FMN or riboflavine. Malate-oxidizing activity was inhibited by hematin. The inhibition was prevented by imidazole or globin.
o
-Phenanthroline, 8-hydroxy quinoline, α,α'-dipyridyl, and
p
-chloromercuribenzoate inhibited malate oxidation. Amytal markedly inhibited oxidation of malate in the presence of oxygen, phenazine methosulfate, or dichlorophenolindophenol, but not in the presence of ferricyanide. The results suggest that the malic dehydrogenase of
A. xylinum
is a FAD enzyme, which contains an ironbinding site essential for its activity. Nonheme iron and sulfhydro groups are possibly involved in enzyme activity. The malic dehydrogenase is functionally linked to the cytochrome chain.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference34 articles.
1. Oxidative phosphorylation in fractional bacterial systems. XII. The properties of malate-vitamin K reductase;ASAN;Biochem. Biophys. Res. Commun.,1963
2. BENTLEY It. 1963. Glucose oxidase p. 567-586. In P. 1). Boier H. Lardy and K. Myrbaek [ed.] The enzvmes vol. 7. Academic press Inc. New York.
3. MeItabolism of dicarboxylic acids in Acetobacter xylinnim;BENZIMAN M.;J. Bacteriol.,1964
4. Synthesis of cellulose from pyruvate by succinate-grown cells of A.cetobacter xylinian;BENZIMAN M.;J. Bacteriol.,1962
5. The isolation of flavin nucleotides;DIMANT E., D.;J. Am. Chem. Soc.,1952
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