Affiliation:
1. Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
Abstract
Benziman, Moshe
(The Hebrew University of Jerusalem, Jerusalem, Israel),
and A. Abeliovitz
. Metabolism of dicarboxylic acids in
Acetobacter xylinum
. J. Bacteriol.
87:
270–277. 1964.—During the oxidation of fumarate or
l
-malate by whole cells or extracts of
Acetobacter xylinum
grown on succinate, a keto acid accumulated in the medium in considerable amounts. This acid was identified as oxaloacetic acid (OAA). No accumulation of OAA was observed when succinate served as substrate. These phenomena could be explained by the kinetics of malate, succinate, and OAA oxidation. OAA did not inhibit malate oxidation, even when present at high concentrations. When cells were incubated with OAA or fumarate in the presence of C
14
O
2
, only the beta-carboxyl of residual OAA was found to be labeled. Evidence was obtained indicating that nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) are not directly involved in malate oxidation by cell-free extracts. The results suggest that malate oxidation in
A. xylinum
is irreversible, and is catalyzed by an enzyme which is not NAD- or NADP-linked.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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