Affiliation:
1. Division of Molecular Biology, U.S. FDA, Laurel, Maryland, USA
2. Division of Microbiology, U.S. FDA, College Park, Maryland, USA
Abstract
ABSTRACT
Serotyping
Escherichia coli
is a cumbersome and complex procedure due to the existence of large numbers of O- and H-antigen types. It can also be unreliable, as many Shiga toxin-producing
E. coli
(STEC) strains isolated from fresh produce cannot be typed by serology or have only partial serotypes. The FDA
E. coli
identification (FDA-ECID) microarray, designed for characterizing pathogenic
E. coli
, contains a molecular serotyping component, which was evaluated here for its efficacy. Analysis of a panel of 75 reference
E. coli
strains showed that the array correctly identified the O and H types in 97% and 98% of the strains, respectively. Comparative analysis of 73 produce STEC strains showed that serology and the array identified 37% and 50% of the O types, respectively, and that the array was able to identify 16 strains that could not be O serotyped. Furthermore, the array identified the H types of 97% of the produce STEC strains compared to 65% by serology, including six strains that were mistyped by serology. These results show that the array is an effective alternative to serology in serotyping environmental
E. coli
isolates.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
31 articles.
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