Argon detector: alternative detection system for gas-liquid chromatographic analysis of short-chain organic acids

Abstract

Two detection systems for gas-liquid chromatography were compared for the identification of metabolic end products (short-chain organic acids) from anaerobic bacteria. Argon and flame ionization detectors were connected in series with inert argon as the carrier gas for analysis of 35 stock strains and 148 clinical isolates. There was an excellent correlation between the argon and flame ionization chromatograms, and both were readily comparable to the thermal conductivity detector tracings published in the Virginia Polytechnic Institute manual. The linear response of the argon detector was examined by analyzing twofold serial dilutions of the short-chain organic acids and comparing the results to the very linear flame ionization detector. The argon detector was found to react with sufficient linearity within the organic acid concentration range normally obtained from anaerobic bacterial broth cultures. The argon detector, therefore, appears to be a viable alternative to the flame ionization and thermal conductivity detector systems in the identification of anaerobic organisms.