Affiliation:
1. National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan
Abstract
ABSTRACT
Certain
rpsL
(which encodes the ribosomal protein S12) mutations that confer resistance to streptomycin markedly activate the production of antibiotics in
Streptomyces
spp. These
rpsL
mutations are known to be located in the two conserved regions within the S12 protein. To understand the roles of these two regions in the activation of silent genes, we used site-directed mutagenesis to generate eight novel mutations in addition to an already known (K88E) mutation that is capable of activating antibiotic production in
Streptomyces lividans
. Of these mutants, two (L90K and R94G) activated antibiotic production much more than the K88E mutant. Neither the L90K nor the R94G mutation conferred an increase in the level of resistance to streptomycin and paromomycin. Our results demonstrate the efficacy of the site-directed mutagenesis technique for strain improvement.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
40 articles.
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