Affiliation:
1. Department of Genetics and Biotechnology, Faculty of Biology, Ivan Franko National University of Lviv, Lviv, Ukraine
Abstract
Abstract:
Species within the actinobacterial genus Streptomyces represent one of the most gifted natural chemists in the microbial world. Their specialized metabolites attract the interest of the pharmaceutical industry as a source of novel drugs. A majority of these molecules pose an insur-mountable challenge for economically justified production via chemical synthesis. Therefore, sub-merged fermentation-based isolation of such molecules often remains the only viable way to obtain them. This in turn fuels interest in process development programs aiming to maximize the yield of specialized metabolite per volume unit of fermentation medium. Along with the optimization of the medium and the fermentation mode itself, strain improvement remains an important part of an over-all process development endeavor. An improved strain can be generated via application of tradition-al approaches of selection for random or induced mutants and genomics-enabled genetic engineer-ing methods. Here I focus on a specific class of mutations with the gene rpsL for ribosomal protein S12, which often confer resistance to streptomycin in bacteria and upregulate specialized metabo-lism in Streptomyces. The review will portray the evolution of our understanding of the mechanisms behind rpsL mutations, as well as how technological advances change the way these mutations are introduced into the genomes of interest.
Funder
Ministry of Education and Science of Ukraine
National Research Foundation of Ukraine
Publisher
Bentham Science Publishers Ltd.
Subject
Applied Microbiology and Biotechnology,Bioengineering,Biotechnology
Reference102 articles.
1. Antimicrobial resistance in the age of COVID-19. Nat Microbiol 2020,5(6),779
2. Carpouron J.E.; de Hoog S.; Gentekaki E.; Hyde K.D.; Emerging animal-associated fungal diseases. J Fungi 2022,8(6),611
3. Ribeiro da Cunha B.; Fonseca L.P.; Calado C.R.C.; Antibiotic discovery: Where have we come from, where do we go? Antibiotics 2019,8(2),45
4. Gratia A.; Dath S.; Proprietes bacteriolytiques de certaines moisissures. Compt Rend Soc Biol 1924,91,1442-1443
5. Welsch M.; Bacteriostatic and bacteriolytic properties of actinomycetes. J Bacteriol 1942,44(5),571-588