Evaluation of Molecular-Beacon, TaqMan, and Fluorescence Resonance Energy Transfer Probes for Detection of Antibiotic Resistance-Conferring Single Nucleotide Polymorphisms in Mixed Mycobacterium tuberculosis DNA Extracts-Reference-Cited by-同舟云学术

Evaluation of Molecular-Beacon, TaqMan, and Fluorescence Resonance Energy Transfer Probes for Detection of Antibiotic Resistance-Conferring Single Nucleotide Polymorphisms in Mixed Mycobacterium tuberculosis DNA Extracts

Published:2006-10 Issue:10 Volume:44 Page:3826-3829
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Yesilkaya Hasan¹,Meacci Francesca²,Niemann Stefan³,Hillemann Doris³,Rüsch-Gerdes Sabine³,Barer Michael R.¹,Andrew Peter W.¹,Oggioni Marco R.²,

Affiliation:

1. Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom

2. Laboratorio di Microbiologia Molecolare e Biotecnologia, Dipartimento di Biologia Molecolare, Universit à di Siena Policlinico Le Scotte (lotto 5, piano 1), 53100 Siena, Italy

3. National Reference Center for Mycobacteria, Forschungszentrum, Borstel, Germany

Abstract

ABSTRACT The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and the ratio of alleles determine the detection sensitivity of the probe systems.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00225-06

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