Rapid Identification of Staphylococcus aureus and the mecA Gene from BacT/ALERT Blood Culture Bottles by Using the LightCycler System-Reference-Cited by-同舟云学术

Rapid Identification of Staphylococcus aureus and the mecA Gene from BacT/ALERT Blood Culture Bottles by Using the LightCycler System

Published:2002-07 Issue:7 Volume:40 Page:2659-2661
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Shrestha Nabin K.¹²,Tuohy Marion J.¹,Hall Gerri S.¹,Isada Carlos M.²,Procop Gary W.¹

Affiliation:

1. Section of Clinical Microbiology

2. Division of Infectious Diseases, The Cleveland Clinic Foundation, Cleveland, Ohio

Abstract

ABSTRACT One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.7.2659-2661.2002

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