Rapid Identification of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR-Reference-Cited by-同舟云学术

Rapid Identification of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR

Published:2000-06 Issue:6 Volume:38 Page:2429-2433
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Reischl Udo¹,Linde Hans-Jörg¹,Metz Michaela¹,Leppmeier Birgit¹,Lehn Norbert¹

Affiliation:

1. Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany

Abstract

ABSTRACT A duplex LightCycler PCR assay targeting the mecA gene and a Staphylococcus aureus -specific marker was used to test 165 S. aureus strains and 80 strains of other bacterial species. Within an assay time of 60 min plus 10 min for sample preparation, S. aureus as well as the presence or absence of the mecA gene was correctly identified.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.6.2429-2433.2000

Reference23 articles.

1. Methicillin-resistant staphylococci;Barber M.;J. Clin. Pathol.,1961

2. Rapid detection of the mecA gene in methicillin resistant staphylococci using a colorimetric cycling probe technology;Bekkaoui F.;Diagn. Microbiol. Infect. Dis.,1999

3. Coagulase-negative staphylococci resistant to beta-lactam antibiotics in vivo produce penicillin-binding protein 2a

4. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications

5. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus

Cited by 167 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A Rapid and Robust DNA Extraction Method for PCR-Based Diagnosis of V. cholerae;Qeios;2024-05-28

2. Hygiene practices and antibiotic resistance among dental and medical students: a comparative study;Infection;2024-03-22

3. Bioremediation potential of cadmium-resistant bacteria isolated from water samples of rivulet Holy Kali Bein, Punjab, India;Bioremediation Journal;2023-11-24

4. Development and utilization of analytical methods for rapid GM detection in processed food products: a case study for regulatory requirement;Journal of Plant Biochemistry and Biotechnology;2023-02-21

5. Development and Utilization of Analytical Methods for Rapid GM Detection in Processed Food Products: A Case Study for Regulatory Requirement;2022-10-31