Aminopeptidase C of Aspergillus niger Is a Novel Phenylalanine Aminopeptidase-Reference-Cited by-同舟云学术

Aminopeptidase C of Aspergillus niger Is a Novel Phenylalanine Aminopeptidase

Published:2003-02 Issue:2 Volume:69 Page:1246-1250
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Basten Daniëlle E. J. W.¹,Dekker Peter J. T.²,Schaap Peter J.¹

Affiliation:

1. Laboratory of Microbiology, Wageningen University, 6703 HA Wageningen

2. DSM Food Specialties, 2600 MA Delft, The Netherlands

Abstract

ABSTRACT A novel enzyme with a specific phenylalanine aminopeptidase activity (ApsC) from Aspergillus niger (CBS 120.49) has been characterized. The derived amino acid sequence is not similar to any previously characterized aminopeptidase sequence but does share similarity with some mammalian acyl-peptide hydrolase sequences. ApsC was found to be most active towards phenylalanine β-naphthylamide (F-βNA) and phenylalanine para -nitroanilide (F- p NA), but it also displayed activity towards other amino acids with aromatic side chains coupled to βNA; other amino acids with nonaromatic side chains coupled to either p NA or βNA were not hydrolyzed or were poorly hydrolyzed. ApsC was not able to hydrolyze N -acetylalanine- p NA, a substrate for acyl-peptide hydrolases.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.69.2.1246-1250.2003

Reference26 articles.

1. Achstetter, T., C. Ehmann, and D. H. Wolf. 1983. Proteolysis in eucaryotic cells: aminopeptidases and dipeptidyl aminopeptidases of yeast revisited. Arch. Biochem. Biophys.226:292-305.

2. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

3. Atlan, D., C. Gilbert, B. Blanc, and R. Portalier. 1994. Cloning, sequencing and characterization of the pepIP gene encoding a proline iminopeptidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397. Microbiology140:527-535.

4. Basten, D. E. J. W., J. Visser, and P. J. Schaap. 2001. Lysine aminopeptidase of Aspergillus niger. Microbiology147:2045-2050.

5. Bateman, A., E. Birney, R. Durbin, S. R. Eddy, R. D. Finn, and E. L. L. Sonnhammer. 1999. Pfam 3.1: 1313 multiple alignments and profile HMMs match the majority of proteins. Nucleic Acids Res.27:260-262.

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