Affiliation:
1. Department of Agricultural Chemistry, Kyushu University, Fukuoka 812, Japan
Abstract
A newly isolated bacterium, identified as
Bacillus subtilis
65, was found to produce raw-starch-digesting α-amylase. The electrophoretically homogeneous preparation of enzyme (molecular weight, 68,000) digested and solubilized raw corn starch to glucose and maltose with small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose. This enzyme was different from other amylases and could digest raw potato starch almost as fast as it could corn starch, but it showed no adsorbability onto any kind of raw starch at any pH. The mixed preparation with
Endomycopsis
glucoamylase synergistically digested raw potato starch to glucose at 30°C. The raw-potato-starch-digesting α-amylase showed strong digestibility to small substrates, which hydrolyzed maltotriose to maltose and glucose, and hydrolyzed
p
-nitrophenyl maltoside to
p
-nitrophenol and maltose, which is different from the capability of bacterial liquefying α-amylase.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference8 articles.
1. Bucl hanan R. E. and N. E. Gibbons (ed.). 1974. Bergey's man iual of determinative bacteriology 8th ed. The Williams & WilL kins Co. Baltimore.
2. Flor P. Q. and S. Hayashida. 1983. Production and characteristic s of raw starch-digesting glucoamylase 0 from a protease
3. Martin R. G. and B. N. Ames. 1961. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. 236:1372-1379.
4. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases;Siegel L. M.;Biochim. Biophys. Acta,1966
5. Sugimoto Y. T. Takaya and H. Fuwa. 1975. In vivo digestion of starch granules of potato yamanoimo high-amylose corn and
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