Cloning and heterologous expression of the entire set of structural genes for nikkomycin synthesis from Streptomyces tendae Tü901 in Streptomyces lividans-Reference-Cited by-同舟云学术

Cloning and heterologous expression of the entire set of structural genes for nikkomycin synthesis from Streptomyces tendae Tü901 in Streptomyces lividans

Published:1996-02 Issue:4 Volume:178 Page:1216-1218
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bormann C¹,Möhrle V¹,Bruntner C¹

Affiliation:

1. Mikrobielle Genetik, Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, Germany.

Abstract

A genomic library from Streptomyces tendae raised in shuttle cosmid vector pKC505 was screened with a previously isolated 8-kb DNA fragment containing the orfP1 gene, which is involved in nikkomycin biosynthesis. The entire set of structural genes for nikkomycin synthesis was heterologously expressed in S. lividans TK23 by introducing recombinant cosmids p24/32 and p9/43-2, carrying inserts of about 31 and 27 kb, respectively, overlapping by 15 kb. S. lividans transformants synthesized nikkomycins X, Z, I, and J, which were identified by high-pressure liquid chromatography analyses of culture filtrates.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.178.4.1216-1218.1996

Reference19 articles.

1. Metabolic products of microorganisms. 228. New nikkomycins produced by mutants of Streptomyces tendae;Bormann C.;J. Antibiot.,1985

2. Isolation of Streptomyces tendae mutants with an altered nikkomycin spectrum;Bormann C.;J. Antibiot.,1989

3. Metabolic products of microorganisms. 181. Chitin synthetase from fungi, a test model for substances with insecticidal properties;Brillinger G. U.;Arch. Microbiol.,1979

4. Stoffwechselprodukte von Mikroorganismen. 154. Mitteilung. Nikkomycin, ein neuer Hemmstoff der Chitinsynthese bei Pilzen;Dähn U.;Arch. Microbiol.,1976

5. The biosynthesis of nikkomycin X from histidine in Streptomyces tendae;Evans D. R.;Tetrahedron Lett.,1995

Cited by 50 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Mechanisms of antifungal resistance;Molecular Medical Microbiology;2024

2. Identification and characterization of enzymes involved in the biosynthesis of pyrimidine nucleoside antibiotics;Natural Product Reports;2021

3. Natural Products Incorporating Pyrimidine Nucleosides;Comprehensive Natural Products III;2020

4. The Chemistry of Purine Nucleoside-Based Antibiotics;Comprehensive Natural Products III;2020

5. C-Nucleoside Formation in the Biosynthesis of the Antifungal Malayamycin A;Cell Chemical Biology;2019-04