Phylogenetic and Biological Characterization of Newcastle Disease Virus Isolates from Pakistan

Author:

Khan Taseer Ahmed1,Rue Cary A.2,Rehmani Shafqat Fatima1,Ahmed Ayaz1,Wasilenko Jamie L.2,Miller Patti J.2,Afonso Claudio L.2

Affiliation:

1. Sindh Poultry Vaccine Center, Animal Science Complex, Korangi, Karachi, Pakistan

2. USDA-ARS Southeast Poultry Research Laboratory, 934 College Station Rd., Athens, Georgia 30605

Abstract

ABSTRACT Eight Newcastle disease virus isolates from Pakistan were sequenced and characterized. A PCR matrix gene assay, designed to detect all avian paramyxovirus 1, did not detect four of the isolates. A new matrix gene test that detected all isolates was developed. Phylogenetic analysis and pathotyping confirmed that virulent viruses of different genotypes are circulating in Pakistan.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

1. Alexander, D. J. 1998. Newcastle disease virus and other avian paramyxoviruses, p. 156-163. In D. E. Swayne et al. (ed.), A laboratory manual for the isolation and identification of avian pathogens, 4th ed. American Association of Avian Pathologists, Kennett Square, PA.

2. Alexander, D. J. 2008. Newcastle disease, other avian paramyxoviruses, and pneumovirus infections, p. 75-115. In Y. M. Saif (ed.), Diseases of poultry, 12th ed. Blackwell, Ames, IA.

3. Kim, L. M., C. L. Afonso, and D. L. Suarez. 2006. Effect of probe-site mismatches on detection of virulent Newcastle disease viruses using a fusion-gene real-time reverse transcription polymerase chain reaction test. J. Vet. Diagn. Invest.18:519-528.

4. Phylogenetic Diversity among Low-Virulence Newcastle Disease Viruses from Waterfowl and Shorebirds and Comparison of Genotype Distributions to Those of Poultry-Origin Isolates

5. Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR

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