Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR-Reference-Cited by-同舟云学术

Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR

Published:2007-04 Issue:4 Volume:45 Page:1310-1314
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kim L. Mia¹,King Daniel J.¹,Suarez David L.¹,Wong Chun W.²,Afonso Claudio L.¹

Affiliation:

1. USDA-ARS Southeast Poultry Research Laboratory, 934 College Station Rd., Athens, Georgia 30605

2. Tai Lung Veterinary Laboratory, Agriculture, Fisheries and Conservation Department, Lin Tong Mei, Sheung Shui, NT, Hong Kong, SAR, People's Republic of China

Abstract

ABSTRACT Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02594-06

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