Scarless Removal of Large Resistance Island AbaR Results in Antibiotic Susceptibility and Increased Natural Transformability in Acinetobacter baumannii-Reference-Cited by-同舟云学术

Scarless Removal of Large Resistance Island AbaR Results in Antibiotic Susceptibility and Increased Natural Transformability in Acinetobacter baumannii

Published:2020-09-21 Issue:10 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Godeux Anne-Sophie¹²,Svedholm Elin¹,Lupo Agnese³,Haenni Marisa³^ORCID,Venner Samuel⁴,Laaberki Maria-Halima¹²^ORCID,Charpentier Xavier¹^ORCID

Affiliation:

1. CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, CNRS UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Villeurbanne, France

2. Université de Lyon, VetAgro Sup, Marcy-l'Étoile, France

3. Unité Antibiorésistance et Virulence Bactériennes, Université de Lyon—ANSES Site de Lyon, Lyon, France

4. Laboratoire de Biométrie et Biologie Evolutive, CNRS UMR5558, Université Claude Bernard Lyon 1, Villeurbanne, France

Abstract

With a great diversity in gene composition, including multiple putative antibiotic resistance genes, AbaR islands are potential contributors to multidrug resistance in Acinetobacter baumannii . However, the effective contribution of AbaR to antibiotic resistance and bacterial physiology remains elusive. To address this, we sought to accurately remove AbaR islands and restore the integrity of their insertion site. To this end, we devised a versatile scarless genome editing strategy.

Funder

Agence Nationale de la Recherche

Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail

VetAgro Sup

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00951-20

Reference31 articles.

1. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii