Global analysis of the RNA-RNA interactome inAcinetobacter baumanniiAB5075 uncovers a small regulatory RNA repressing the virulence-related outer membrane protein CarO

Author:

Hamrock Fergal J.ORCID,Ryan DanielORCID,Shaibah Ali,Ershova Anna S.ORCID,Mogre AalapORCID,Sulimani Maha M.,Reichardt Sarah,Hokamp KarstenORCID,Westermann Alexander J.ORCID,Kröger CarstenORCID

Abstract

ABSTRACTAcinetobacter baumanniiis an opportunistic Gram-negative pathogen that infects critically ill patients. The emergence of antimicrobial resistantA. baumanniihas exacerbated the need to functionally characterise environmental adaptation, antibiotic resistance and pathogenicity of this organism and their genetic regulators to inform intervention strategies. Critical to rapid adaptation to changing environments in bacteria are small regulatory RNAs (sRNAs), however, the role that sRNAs play in the biology ofA. baumanniiis poorly understood. To assess the regulatory function of sRNAs and to uncover their RNA interaction partners inA. baumannii, we employed an RNA proximity ligation and sequencing method (Hi-GRIL-seq) in three different environmental conditions. We found that 40 sRNA candidates were ligated to sRNA-RNA chimeric sequencing reads, suggesting that sRNA-mediated gene regulation is pervasive inA. baumanniiand that sRNAs act as direct regulators of mRNA molecules through antisense base-pairing. In-depth characterisation uncovered the sRNA Aar to be a post-transcriptional regulator of four mRNA targets including that of the outer membrane protein CarO and the siderophore receptor BfnH. We show that Aar initiates base-pairing with these mRNA molecules using a conserved seed region of nine nucleotides, sequestering the ribosome binding sites and inhibiting translation. Aar is differentially expressed in response to multiple stress stimuli suggesting a role in fine-tuning translation of the Aar-target molecules inA. baumanniiunder hostile conditions. Together, our study provides mechanistic insights into sRNA-mediated gene expression control inA. baumanniiand represents a valuable resource for future RNA-centric research endeavours in this ESKAPE pathogen.

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3