Aspergillus fumigatus C-5 Sterol Desaturases Erg3A and Erg3B: Role in Sterol Biosynthesis and Antifungal Drug Susceptibility-Reference-Cited by-同舟云学术

Aspergillus fumigatus C-5 Sterol Desaturases Erg3A and Erg3B: Role in Sterol Biosynthesis and Antifungal Drug Susceptibility

Published:2006-02 Issue:2 Volume:50 Page:453-460
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Alcazar-Fuoli Laura¹,Mellado Emilia¹,Garcia-Effron Guillermo¹,Buitrago Maria J.¹,Lopez Jordi F.²,Grimalt Joan O.²,Cuenca-Estrella J. Manuel¹,Rodriguez-Tudela Juan L.¹

Affiliation:

1. Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

2. Department of Environmental Chemistry, I.C.E.R.-C.S.I.C., Jordi Girona, 18, 08034 Barcelona, Spain

Abstract

ABSTRACT Two erg3 genes encoding C-5 sterol desaturase enzymes (Erg3A and Erg3B) in Aspergillus fumigatus were characterized with respect to their nucleotide sequences and null mutant phenotypes. Targeted disruption of the erg3A and erg3B genes and a double gene knockout, erg3A − erg3B − , showed that they are not essential for A. fumigatus viability. Mutant phenotypes clearly showed that Erg3B is a C-5 sterol desaturase, but no apparent role for Erg3A in A. fumigatus ergosterol biosynthesis was found. Susceptibility to amphotericin B, itraconazole, fluconazole, voriconazole, and ketoconazole was not altered in isolates in which erg3A and erg3B were knocked out alone and in combination.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.50.2.453-460.2006

Reference50 articles.

1. Arthington, B. A., L. G. Bennett, P. L. Skatrud, C. J. Guynn, R. J. Barbuch, C. E. Ulbright, and M. Bard. 1991. Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase. Gene102:39-44.

2. Quantitation of Candida albicans Ergosterol Content Improves the Correlation between In Vitro Antifungal Susceptibility Test Results and In Vivo Outcome after Fluconazole Treatment in a Murine Model of Invasive Candidiasis

3. Bard, M., N. D. Lees, T. Turi, D. Craft, L. Cofrin, R. Barbuch, C. Koegel, and J. C. Loper. 1993. Sterol synthesis and viability of erg11 (cytochrome P450 lanosterol demethylase) mutations in Saccharomyces cerevisiae and Candida albicans. Lipids28:963-967.

4. Chryssanthou, E. 1997. In vitro susceptibility of respiratory isolates of Aspergillus species to itraconazole and amphotericin B acquired resistance to itraconazole. Scand. J. Infect. Dis.29:509-512.

5. Clinical and Laboratory Standards Institute. 2005. Reference method for broth dilution antifungal susceptibility. Testing of filamentous fungi. Approved standard document M38-A. Clinical and Laboratory Standards Institute Wayne Pa.

Cited by 48 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Toxic eburicol accumulation drives the antifungal activity of azoles against Aspergillus fumigatus;Nature Communications;2024-07-26

2. Toxic eburicol accumulation drives the antifungal activity of azoles againstAspergillus fumigatus;2024-03-02

3. Ergosterol Is Critical for Sporogenesis in Cryptococcus neoformans;Journal of Fungi;2024-01-26

4. The Microevolution of Antifungal Drug Resistance in Pathogenic Fungi;Microorganisms;2023-11-13

5. The sterol C-24 methyltransferase encoding gene,erg6, is essential for viability ofAspergillusspecies;2023-08-09