Affiliation:
1. Department of Microbiology, Cornell University, Ithaca, New York 14853-8101
Abstract
ABSTRACT
A Tn
7
donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium
Bacillus subtilis
. This new mini-Tn
7
, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream of the transposon. We demonstrate that mTn7SX inserts are obtained at a high frequency and occur randomly throughout the
B. subtilis
genome. The utility of this system was demonstrated by the selection of mutants with increased resistance to the antibiotic fosfomycin or duramycin.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
13 articles.
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