Monitoring the Antiviral Effect of Alpha Interferon on Individual Cells-Reference-Cited by-同舟云学术

Monitoring the Antiviral Effect of Alpha Interferon on Individual Cells

Published:2007-08-15 Issue:16 Volume:81 Page:8814-8820
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Kim Chon Saeng¹,Jung Jong Ha¹,Wakita Takaji²,Yoon Seung Kew³,Jang Sung Key¹

Affiliation:

1. PBC, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea

2. Department of Virology II, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan

3. Department of Internal Medicine, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea

Abstract

ABSTRACT An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-α) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-α were observed among HCV-containing cells.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02824-06

Reference18 articles.

1. Alter, H. J., and L. B. Seeff. 2000. Recovery, persistence, and sequelae in hepatitis C virus infection: a perspective on long-term outcome. Semin. Liver Dis.20:17-35.

2. Bartenschlager, R., and V. Lohmann. 2001. Novel cell culture systems for the hepatitis C virus. Antiviral Res.52:1-17.

3. Bartenschlager, R., and T. Pietschmann. 2005. Efficient hepatitis C virus cell culture system: what a difference the host cell makes. Proc. Natl. Acad. Sci. USA102:9739-9740.

4. Mutations in the 5′ Nontranslated Region of Bovine Viral Diarrhea Virus Result in Altered Growth Characteristics

5. Peginterferon Alfa-2a plus Ribavirin for Chronic Hepatitis C Virus Infection

Cited by 34 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Current status and future development of infectious cell-culture models for the major genotypes of hepatitis C virus: Essential tools in testing of antivirals and emerging vaccine strategies;Antiviral Research;2018-10

2. Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production;International Journal of Biological Sciences;2018

3. The Intracellular Cholesterol Transport Inhibitor U18666A Inhibits the Exosome-Dependent Release of Mature Hepatitis C Virus;Journal of Virology;2016-12-15

4. The hepatitis C virus protein NS3 suppresses TNF-α–stimulated activation of NF-κB by targeting LUBAC;Science Signaling;2015-11-17

5. A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus;Scientific Reports;2015-03-09