Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory-Reference-Cited by-同舟云学术

Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory

Published:2020-09-16 Issue:4 Volume:33 Page:
ISSN:0893-8512
Container-title:Clinical Microbiology Reviews
language:en
Short-container-title:Clin Microbiol Rev

Author:

Church Deirdre L.¹²^ORCID,Cerutti Lorenzo³,Gürtler Antoine⁴⁵,Griener Thomas¹,Zelazny Adrian⁶,Emler Stefan⁴⁵

Affiliation:

1. Departments of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada

2. Department of Medicine, University of Calgary, Calgary, Alberta, Canada

3. Health 2030 Genome Center, Foundation Campus Biotech Geneva, Geneva, Switzerland

4. SmartGene Services, Lausanne, Switzerland

5. Department of Laboratory Medicine, University of Lausanne, Lausanne, Switzerland

6. National Institutes of Health, Bethesda, Maryland, USA

Abstract

This review provides a state-of-the-art description of the performance of Sanger cycle sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical microbiology laboratory. A detailed description of the technology and current methodology is outlined with a major focus on proper data analyses and interpretation of sequences. The remainder of the article is focused on a comprehensive evaluation of the application of this method for identification of bacterial pathogens based on analyses of 16S multialignment sequences.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,General Immunology and Microbiology,Epidemiology

Link

https://journals.asm.org/doi/pdf/10.1128/CMR.00053-19

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