Affiliation:
1. Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Abstract
Approximately 140 mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on media lacking fixed nitrogen (Fix-) were isolated after mutagenesis with diethyl sulfate and penicillin enrichment. A large cosmid library of wild-type Anabaena sp. strain PCC 7120 DNA was constructed in a mini-RK-2 shuttle vector, and seven mutants representing several morphologically abnormal heterocyst phenotypes were complemented. One of these mutants, 216, failed to differentiate heterocysts. All of these mutants except 216 reduced acetylene under anaerobic conditions, indicating that they are not defective in nitrogen fixation per se. Several cosmids were isolated from each complemented mutant and in most cases showed similar restriction patterns. Comparisons of the complementing cosmids from mutant 216 and two other phenotypically distinct mutants by restriction enzyme analysis identified a common region. This region, when present in either a cosmid or a 9.5-kb NheI subclone, is capable of efficiently complementing all three mutants. A 2.4-kb subclone of this region complements mutant 216 only.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference21 articles.
1. Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120;Bancroft I.;J. Bacteriol.,1989
2. Carr N. G. 1983. Biochemical aspects of heterocyst differentiation and function p. 265-280. In G. C. Papageorgiou and L. Packer (ed.) Photosynthetic prokaryotes. Elsevier Science Publishing Inc. New York.
3. Conditions for mutagenesis of the nitrogen-fixing cyanobacterium Anabaena variabilis;Chapman J. S.;J. Gen. Microbiol.,1987
4. Isolation, sequence and expression of two members of the 32 kd thylakoid membrane gene family from the cyanobacterium Anabaena 7120;Curtis S. E.;Plant Mol. Biol.,1984
5. Conjugal transfer of DNA to cyanobacteria;Elhai J.;Methods Enzymol.,1988
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