Abstract
FurC (PerR, Peroxide Response Regulator) fromAnabaenasp. PCC 7120 (also known asNostocsp. PCC 7120) is a master regulator engaged in the modulation of relevant processes including the response to oxidative stress, photosynthesis and nitrogen fixation. Previous differential gene expression analysis of afurC-overexpressing strain (EB2770FurC) allowed the inference of a putative FurC DNA-binding consensus sequence. In the present work, more data concerning the regulon of the FurC protein were obtained through the searching of the putative FurC-box in the wholeAnabaenasp. PCC 7120 genome. The total amount of novel FurC-DNA binding sites found in the promoter regions of genes with known function was validated by electrophoretic mobility shift assays (EMSA) identifying 22 new FurC targets. Some of these identified targets display relevant roles in nitrogen fixation (hetRandhgdC) and carbon assimilation processes (cmpR,glgP1andopcA), suggesting that FurC could be an additional player for the harmonization of carbon and nitrogen metabolisms. Moreover, differential gene expression of a selection of newly identified FurC targets was measured by Real Time RT-PCR in thefurC-overexpressing strain (EB2770FurC) comparing toAnabaenasp. PCC 7120 revealing that in most of these cases FurC could act as a transcriptional activator.
Funder
Ministerio de Ciencia e Innovación
Gobierno de Aragón
Publisher
Public Library of Science (PLoS)