Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Viruses-Reference-Cited by-同舟云学术

Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Viruses

Published:1999-12 Issue:12 Volume:37 Page:4139-4141
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Williams K.¹,Blake S.¹,Sweeney A.¹,Singer J. T.¹,Nicholson B. L.¹

Affiliation:

1. Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, Maine 04469

Abstract

ABSTRACT A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.12.4139-4141.1999

Reference24 articles.

1. Absence of infectious hematopoietic necrosis virus in adult sockeye salmon;Amos K. H.;J. Aquat. Anim. Health,1989

2. Polymerase chain reaction (PCR) amplification of a nucleoprotein gene sequence of infectious hematopoietic necrosis virus;Arakawa C. K.;Dis. Aquat. Org.,1990

3. Detection and identification of aquatic birnaviruses by PCR assay

4. Differential diagnosis of fish pathogenic rhabdoviruses by reverse transcriptase-dependent polymerase chain reaction;Bruchhof B.;J. Virol. Methods,1995

5. Use of a group-reactive and other monoclonal antibodies in an enzyme immunodot assay for identification and presumptive serotyping of aquatic birnaviruses

Cited by 62 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Isolation and Identification of Hemorrhagic Septicemia Virus from Shrimp;Springer Protocols Handbooks;2023

2. Simultaneous detection of three important viruses affecting tilapia using a multiplex PCR assay;Journal of Fish Diseases;2022-11-28

3. Establishment and Application of a Multiplex PCR Assay for the Rapid Detection of Rhizoctonia solani Anastomosis Group (AG)-3PT, the Pathogen Causing Potato Black Scurf and Stem Canker;Pathogens;2022-05-29

4. A panoptic review of techniques for finfish disease diagnosis: The status quo and future perspectives;Journal of Microbiological Methods;2022-05

5. Diagnosis of infectious pancreatic necrosis virus (IPNV): Review;Ribogospodarsʹka nauka Ukraïni.;2022-03-31