Optimal Activation of Isopsoralen To Prevent Amplicon Carryover-Reference-Cited by-同舟云学术

Optimal Activation of Isopsoralen To Prevent Amplicon Carryover

Published:1999-01 Issue:1 Volume:37 Page:261-262
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fahle Gary A.¹,Gill Vee J.¹,Fischer Steven H.¹

Affiliation:

1. Microbiology Service, Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508

Abstract

ABSTRACT We compared the efficiencies of activation of the photochemical isopsoralen compound 10 and its resulting amplicon neutralizations under conditions with a UV transilluminator box at room temperature (RT) and a HRI-300 UV photothermal reaction chamber at RT and at 5°C. Our data suggest that use of the HRI-300 reaction chamber at 5°C results in a statistically significantly higher degree of amplicon neutralization.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.1.261-262.1999

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