Molecular Identification of a Nocardiopsis dassonvillei Blood Isolate

Author:

Beau Frédéric1,Bollet Claude1,Coton Thierry2,Garnotel Eric3,Drancourt Michel1

Affiliation:

1. Laboratoire de Bactériologie, Assistance Publique—Hôpitaux de Marseille,1 and

2. Service de Gastro-entérologie2 and

3. Laboratoire de Microbiologie,3Hôpital d’Instruction des Armées Alphonse Laveran, Marseille, France

Abstract

ABSTRACT Nocardiopsis dassonvillei is an environmental aerobic actinomycete seldom isolated in cutaneous and pulmonary infections. We herein report the first N. dassonvillei blood isolate in a patient hospitalized for cholangitis. Although morphological characteristics and biochemical tests allowed a presumptive identification of this isolate, cell wall fatty acid chromatographic analysis confirmed identification at the genus level, and 16S rRNA gene sequencing achieved definite identification. This study illustrates the usefulness of 16S rRNA gene sequencing as a routine method for the identification of actinomycetes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference26 articles.

1. Actinomycetoma caused by Nocardiopsis dassonvillei;Ajello L.;Arch. Dermatol.,1987

2. Numerical classification of Actinomadura and Nocardiopsis;Athalye M.;Int. J. Syst. Bacteriol.,1985

3. Nocardiopsis dassonvillei recovered from a lung biopsy and a possible cause of extrinsic alveolitis;Bernatchez H.;Clin. Microbiol. Newsl.,1991

4. Sur un Streptothrix;Brocq-Rousseau D.;Rev. Bot.,1904

5. Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis;Fischer A.;J. Gen. Microbiol.,1983

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