Affiliation:
1. Department of Veterinary Bacteriology and Public Health, University of Minnesota, St. Paul, Minnesota 55101
Abstract
Sheep erythrocyte ghosts released water-soluble organic phosphorus when treated with purified β-hemolysin. Phospholipid analysis demonstrated that sphingomyelin accounted for 53% of the phospholipids present in sheep erythrocytes. Purified β-hemolysin showed phospholipase C activity when purified ox brain or sheep erythrocyte sphingomyelin was used as substrate. Such studies have also revealed that the disappearance of sphingomyelin from the reaction mixture was accompanied by a comparable increase in the concentration of phosphoryl choline. Thin-layer chromatography of phospholipids, extracted from sheep erythrocytes which had been exposed to β-hemolysin, demonstrated that sphingomyelin was rapidly degraded. Activators of β-hemolysin, such as Mg
++
, enhanced the release of organic phosphorus from erythrocyte ghosts and from sphingomyelin. Inhibitors of β-hemolysin, such as ethylenediaminetetraacetic acid,
p
-chloromercuribenzoate, and iodoacetamide, also inhibited the release of organic phosphorus from erythrocyte ghosts and from sphingomyelin. These studies strongly suggested that β-hemolysin enzymatically degraded the sphingomyelin of the erythrocyte membrane. Such degradation probably resulted in the eventual lysis of the erythrocyte.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
21 articles.
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