Affiliation:
1. Department of Bacteriology, Iowa State University, Ames, Iowa
Abstract
A strain of
Pseudomonas aeruginosa
has been shown to produce an enzyme that lyses viable cells of
Staphylococcus aureus
. The maximal yield of the enzyme was obtained from shake flask cultures of
P. aeruginosa
which were grown for 18 to 22 hr at 37 C in Trypticase Soy Broth. A 333-fold purification of the enzyme was obtained by acetone precipitation of the culture liquor, followed by column chromatography on phosphonic acid cellulose and Bio-Gel P2. The staphylolytic enzyme exhibited maximal activity at 37 C in 0.01
m
sodium phosphate (
p
H 8.5) and was stable at 37 C in the
p
H range of 7.5 to 9.5. The inhibition and stabilization of the enzyme by various organic and inorganic materials was investigated. Spheroplasts of
S. aureus
were formed by treating viable cells with the staphylolytic enzyme in 1
m
sucrose or human serum.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
25 articles.
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