Affiliation:
1. Department of Bacteriology and Mycology, Janssen Research Foundation, Beerse, Belgium.
Abstract
In microbroth cultures with RPMI 1640 medium, the growth yield of seven Cryptococcus neoformans isolates was unaffected by augmentation of the normal (0.2%) glucose concentration in the medium to 2%, and the addition of other potential carbon, nitrogen, and vitamin sources to the medium also failed to produce large changes in growth yield. However, macrobroth cultures of C. neoformans in RPMI 1640 that were agitated by rotation in air gave turbidities 6 to 37 times greater than those in identical cultures incubated statically, and similar levels of increase were seen whether the medium contained 0.2 or 2% glucose. Incubation of microplates under an oxygen atmosphere or with agitation by rotation led to an increase of up to twofold in growth turbidity of the yeast. The maximum increase was achieved by incubation with rotation and was dependent on the brand of microplate used. The findings implicate oxygen as a growth-limiting nutrient for C. neoformans. Incubation of microbroth cultures under conditions that enhance oxygen availability for antifungal susceptibility testing purposes may increase the speed of such tests and enhance the determination of MIC endpoints.
Publisher
American Society for Microbiology
Reference4 articles.
1. National Committee for Clinical Laboratory Standards. 1992. Reference method for broth dilution antifungal susceptibility testing of yeasts. Proposed guideline M27-P. National Committee for Clinical Laboratory Standards Villanova Pa.
2. Quantitative microculture system with standardized inocula for strain typing, susceptibility testing, and other physiologic measurements with Candida albicans and other yeasts;Odds F. C.;J. Clin. Microbiol.,1991
3. Antifungal susceptibility testing of Candida spp. by relative growth measurement at single concentrations of antifungal agents;Odds F. C.;Antimicrob. Agents Chemother.,1992
4. Rodriguez de Miranda L. 1984. Cryptococcus Kützing emend. Phaff et Spencer p. 845-872. In N. J. W. Kreger-Van Rij (ed.) The yeasts 3rd ed. Elsevier Science Publishers Amsterdam.
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