Affiliation:
1. Institute of Physics, University of Basel, Basel, Switzerland
Abstract
ABSTRACT
A rapid biosensor for the detection of bacterial growth was developed using micromechanical oscillators coated in common nutritive layers. The change in resonance frequency as a function of the increasing mass on a cantilever array forms the basis of the detection scheme. The calculated mass sensitivity according to the mechanical properties of the cantilever sensor is ∼50 pg/Hz; this mass corresponds to an approximate sensitivity of ∼100
Escherichia coli
cells. The sensor is able to detect active growth of
E. coli
cells within 1 h. The starting number of
E. coli
cells initially attached to the sensor cantilever was, on average, ∼1,000 cells. Furthermore, this method allows the detection of selective growth of
E. coli
within only 2 h by adding antibiotics to the nutritive layers. The growth of
E. coli
was confirmed by scanning electron microscopy. This new sensing method for the detection of selective bacterial growth allows future applications in, e.g., rapid antibiotic susceptibility testing.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
102 articles.
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