Affiliation:
1. Department of Biological Sciences, Northern Illinois University, DeKalb 60115.
Abstract
The formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid (SHCHC), the first identified intermediate in the menaquinone biosynthetic pathway, requires two reactions. They are the decarboxylation of alpha-ketoglutarate by an alpha-ketoglutarate decarboxylase, which results in the formation of succinic semialdehyde-thiamine PPi (TPP) anion, and the addition of the succinic semialdehyde-TPP anion to isochorismate carried out by the enzyme SHCHC synthase. Evidence is provided to support the conclusion that both enzymatic activities are encoded by an extended menD gene which is capable of generating a bifunctional 69-kDa protein. Consistent with the requirement for TPP in the decarboxylation of alpha-ketoglutarate, the translated amino acid sequence contains the characteristic TPP-binding motif present in all well-characterized TPP-requiring enzymes.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference38 articles.
1. Regulation of (x-ketoglutarate dehydrogenase formation in Escherichia coli;Amarasingham C. R.;J. Biol. Chem.,1965
2. Sequence and organization of the human mitochondrial genome;Anderson S.;Nature (London),1981
3. Biosynthesis of vitamin K (menaquinone) in bacteria;Bentley R.;Microbiol. Rev.,1982
4. Bentley R. and R. Meganathan. 1987. Biosynthesis of the isoprenoid quinones ubiquinone and menaquinone p. 512-520. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology Washington D.C.
5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
Cited by
59 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献