Bacteriophage Typing of Proteus mirabilis, Proteus vulgaris , and Proteus morganii

Author:

Schmidt William C.1,Jeffries Charles D.1

Affiliation:

1. Department of Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201

Abstract

A bacteriphage typing scheme for differentiating Proteus isolated from clinical specimens was developed. Twenty-one distinct patterns of lysis were seen when 15 bacteriophages isolated on 8 Proteus mirabilis , 1 P. vulgaris , and 1 P. morganii were used to type 162 of 189 (85.7%) P. mirabilis and P. vulgaris isolates. Seven phages isolated on 3 P. morganii were used to type 13 of 19 (68.4%) P. morganii isolates. Overall, 84.1% of the 208 isolates were lysed by at least 1 phage at routine test dilution (RTD) or 1,000 × RTD. Fifty isolates, retyped several weeks after the initial testing, showed no changes in lytic patterns. The phages retained their titers after storage at 4 C for several months. A computer analysis of the data showed that there was no relationship between the source of the isolate and bacteriophage type. This bacteriophage typing system may provide epidemiological information on strains involved in human infections.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference29 articles.

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2. Proteus infections in a general hospital. I. Biochemical characteristics and antibiotic susceptibility of the organisms, with special reference to proticine typing and the Dienes phenomenon;Adler J. L.;Ann. Intern. Med.,1971

3. Bacteriophage typing of enteric pathogens and staphylococci and its use in epidemiology;Anderson E. S.;J. Clin. Pathol.,1956

4. Phage typing of staphylococci;Blair J. E.;Bull. W. H. O.,1961

5. Comparison of methods for typing Proteus mirabilis as applied in an epidemiological study of infections in a nursery for newborns;Burke J. P.;Antimicrob. Ag. Chemother.,1971

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