Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells-Reference-Cited by-同舟云学术

Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells

Published:1998-04 Issue:4 Volume:66 Page:1783-1786
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Fu Yue¹,Rieg Günter¹,Fonzi William A.²,Belanger Paul H.¹,Edwards John E.¹³,Filler Scott G.¹³

Affiliation:

1. St. John’s Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 905021;

2. Department of Microbiology and Immunology, Georgetown University, Washington, D.C. 200072; and

3. UCLA School of Medicine, Los Angeles, California 900243

Abstract

ABSTRACT To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae . These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1 . These results indicate that ALS1 may encode a candidal adhesin.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.66.4.1783-1786.1998

Reference13 articles.

1. Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.;Elledge S. J.;Proc. Natl. Acad. Sci. USA,1991

2. Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells

3. Fu Y. S. G. Filler B. J. Spellberg W. A. Fonzi A. S. Ibrahim T. Kanbe M. A. Ghannoum and J. E. Edwards Jr. Cloning and characterization of CAD1/AAF1 a gene from Candida albicans that induces adherence to endothelium when expressed in Saccharomyces cerevisiae . Infect. Immun. in press.

4. Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins

5. Modulation of interactions of Candida albicans and endothelial cells by fluconazole and amphotericin B

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