Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures-Reference-Cited by-同舟云学术

Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures

Published:2015-12 Issue:12 Volume:53 Page:3908-3911
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pasteran Fernando¹,Tijet Nathalie²,Melano Roberto G.²³⁴,Corso Alejandra¹

Affiliation:

1. Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Buenos Aires, Argentina

2. Public Health Ontario Laboratories (PHOL), Toronto, Ontario, Canada

3. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

4. Department of Microbiology, Mt. Sinai Hospital, Toronto, Ontario, Canada

Abstract

ABSTRACT We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPt-direct was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02032-15

Reference11 articles.

1. Rapid Detection of Carbapenemase-producingEnterobacteriaceae

2. Evaluation of the Carba NP Test for Rapid Detection of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

3. Comparison of a Novel, Rapid Chromogenic Biochemical Assay, the Carba NP Test, with the Modified Hodge Test for Detection of Carbapenemase-Producing Gram-Negative Bacilli

4. Evaluation of the Carba NP Test for Carbapenemase Detection

5. Clinical and Laboratory Standards Institute. 2015. Performance standards for antimicrobial susceptibility testing: 25th informational supplement. CLSI M100-S25. Clinical and Laboratory Standards Institute, Wayne, PA.

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