Isolation and Characterization of Sorbitol-Fermenting Shiga Toxin (Verocytotoxin)-Producing Escherichia coli O157:Hßtrains in the Czech Republic

Author:

Bielaszewska Martina1,Schmidt Herbert2,Karmali Mohamed A.3,Khakhria Rasik4,Janda Jan5,Bláhová Kvĕta5,Karch Helge2

Affiliation:

1. Institute of Medical Microbiology, The 2nd Medical Faculty, Charles University,1 and

2. Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg, Germany2; and

3. Division of Microbiology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8,3 and

4. National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario K1A 0L2,4 Canada

5. 1st Clinic of Pediatrics, University Hospital Motol,5 150 06 Prague, Czech Republic;

Abstract

ABSTRACT Two sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157:H− strains were isolated from patients with hemolytic-uremic syndrome in the Czech Republic in 1995. Their phenotypic and genotypic characteristics and genomic DNA fingerprints were identical or closely related to those of SF STEC O157:H− strains isolated in Germany in 1988 to 1997. This indicates that the Czech isolates belong to the SF STEC O157 clone which is widespread in Germany. It is the first finding of the clone outside Germany.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference19 articles.

1. A biotyping scheme for Shiga-like (Vero) toxin-producing Escherichia coli O157 and a list of serological cross-reactions between O157 and other Gram-negative bacteria;Aleksić S.;Zentralbl. Bakteriol.,1992

2. Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli

3. Verocytotoxin-producing Escherichia coli in children with hemolytic uremic syndrome in the Czech Republic;Bielaszewska M.;Clin. Nephrol.,1996

4. Indirect hemagglutination assay for diagnosis of Escherichia coli O157 infection in patients with hemolytic-uremic syndrome

5. Edwards P. R. Ewing W. H. Identification of Enterobacteriaceae 3rd ed. 1972 67 101 Burgess Publishing Minneapolis Minn

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