Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli

Author:

Beutin L1,Montenegro M A1,Orskov I1,Orskov F1,Prada J1,Zimmermann S1,Stephan R1

Affiliation:

1. Robert Koch-Institut des Bundesgesundheitsamtes, Berlin 65, Federal Republic of Germany.

Abstract

Sixty-four verotoxin-producing (VT+) Escherichia coli strains were analyzed for VT1- and VT2-specific DNA sequences and for production of hemolysin. Strains of human origin were of the following serotypes: O157:H7 or H-, O111:H8 or H-, O26:H11, O114:H4, and rough:H7. Strains of serotypes O157:H7, O113:H21, O116:H21, and rough:H- were from cattle, while those of serotype O139:K12:H1 were from pigs. All 64 isolates carried either VT1 or VT2 or both genes. Sixty of the strains (93.8%) were hemolytic (Hly+). The three O139:K12:H1 strains examined produced alpha-hemolysin, as shown by their reaction with the alpha-hemolysin-specific monoclonal antibody h2A and by DNA hybridization with an alpha-hly gene probe. The remaining 57 Hly+ strains (95%) produced a different type of hemolysin (enterohemolysin), which is genetically and serologically unrelated to alpha-hemolysin. The two types of hemolysin are further distinguished by the appearance of the lysis zone on blood agar and by the time interval for the detection of hemolysis. In contrast to alpha-hemolysin, enterohemolysin can be detected only on blood plates containing washed erythrocytes. The frequent association of enterohemolysin with verotoxin production (89%) makes it useful as an epidemiological marker for rapid and simple detection of potential VT+ E. coli.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference40 articles.

1. Six widespread bacterial clones among Escherichia coli Ki isolates;Achtman M.;Infect. Immun.,1983

2. Rapid visual detection of Escherichia coli and Vibrio cholerae heat-labile enterotoxins by nitrocellulose enzyme-linked immunosorbent assay;Beutin L.;J. Clin. Microbiol.,1984

3. Characterization of hemolytic strains of Escherichia coli belonging to classical enteropathogenic 0-serogroups;Beutin L.;Zentralbl. Bakteriol. Hyg. A,1986

4. Enterohemolysin, a new type of hemolysin produced by some strains of enteropathogenic E. coli (EPEC);Beutin L.;Zentralbl. Bakteriol. Hyg. A,1988

5. Detection of enteric pathotypes of Escherichia coli by a hybridization using six DNA probes;Bohnert M. G.;Ann. Inst. Pasteur Microbiol.,1988

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3