Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing-Reference-Cited by-同舟云学术

Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing

Published:2020-10-15 Issue:21 Volume:86 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Thompson Mitchell G.¹²³,Incha Matthew R.¹²⁴,Pearson Allison N.¹²⁴,Schmidt Matthias¹²⁵,Sharpless William A.¹²,Eiben Christopher B.¹²⁶,Cruz-Morales Pablo¹²⁷,Blake-Hedges Jacquelyn M.¹²⁸,Liu Yuzhong¹²,Adams Catharine A.²,Haushalter Robert W.¹²,Krishna Rohith N.¹²,Lichtner Patrick¹²,Blank Lars M.⁵,Mukhopadhyay Aindrila¹²^ORCID,Deutschbauer Adam M.⁴⁹,Shih Patrick M.¹²³¹⁰^ORCID,Keasling Jay D.¹²⁶¹¹¹²^ORCID

Affiliation:

1. Joint BioEnergy Institute, Emeryville, California, USA

2. Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

3. Department of Plant Biology, University of California, Davis, California, USA

4. Department of Plant and Microbial Biology, University of California, Berkeley, California, USA

5. Institute of Applied Microbiology (iAMB), Aachen Biology and Biotechnology (ABBt), RWTH Aachen University, Aachen, Germany

6. Joint Program in Bioengineering, University of California, Berkeley, California, USA

7. Centro de Biotecnología FEMSA, Instituto Tecnológico y de Estudios Superiores de Monterrey, Monterrey, México

8. Department of Chemistry, University of California, Berkeley, California, USA

9. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

10. Environmental and Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

11. Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California, USA

12. Institute for Quantitative Biosciences, University of California, Berkeley, California, USA

Abstract

To engineer novel metabolic pathways into P. putida , a comprehensive understanding of the genetic basis of its versatile metabolism is essential. Here, we provide functional evidence for the putative roles of hundreds of genes involved in the fatty acid and alcohol metabolism of the bacterium. These data provide a framework facilitating precise genetic changes to prevent product degradation and to channel the flux of specific pathway intermediates as desired.

Funder

U.S. Department of Energy

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01665-20

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