CRISPR /Cas9‐enhanced ss DNA recombineering for Pseudomonas putida
Author:
Affiliation:
1. Systems and Synthetic Biology Program Centro Nacional de Biotecnología (CNB‐CSIC) Campus de Cantoblanco 28049 Madrid Spain
Funder
HELIOS Project
ARISYS
EmPowerPutida
MADONNA
InGEMICS-CM
Publisher
Wiley
Subject
Applied Microbiology and Biotechnology,Biochemistry,Bioengineering,Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1111/1751-7915.13453
Reference35 articles.
1. The Ssr protein (T1E_1405) fromPseudomonas putidaDOT-T1E enables oligonucleotide-based recombineering in platform strainP. putidaEM42
2. CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency inPseudomonas putida
3. A broad host range plasmid-based roadmap for ssDNA-based recombineering in Gram-negative bacteria;Aparicio T.;Methods in Molecular Biology, Horizontal Gene transfer,2019
4. MutS interaction with mismatch and alkylated base containing DNA molecules detected by optical biosensor
5. CRISPy-web: An online resource to design sgRNAs for CRISPR applications
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