Confirming Positive Results of Nucleic Acid Amplification Tests (NAATs) for Chlamydia trachomatis : All NAATs Are Not Created Equal

Author:

Schachter J.1,Hook E. W.2,Martin D. H.3,Willis D.4,Fine P.5,Fuller D.6,Jordan J.7,Janda W. M.8,Chernesky M.9

Affiliation:

1. University of California, San Francisco, California

2. University of Alabama, Birmingham, Alabama

3. Louisiana State University Medical Center, New Orleans, Louisiana

4. Florida State Department of Health, Jacksonville, Florida

5. Planned Parenthood Foundation of Houston and Southeast Texas, Houston, Texas

6. Wishard Memorial Hospital, Indianapolis, Indiana

7. Magee-Womens Research Institute, Pittsburgh, Pennsylvania

8. University of Illinois, Chicago, Illinois

9. St. Joseph's Health Care Regional Virology and Chlamydiology Laboratory, Hamilton, Ontario, Canada

Abstract

ABSTRACT The Centers for Disease Control and Prevention recommended confirming positive screening tests for Chlamydia trachomatis when positive predictive values are <90%. It is accepted that less sensitive tests (i.e., culture and immunoassays) should not be used to confirm the results of more sensitive nucleic acid amplification tests (NAATs). We show that the same principle applies when NAATs are used for confirmation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference9 articles.

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2. Reproducibility of Positive Test Results in the BDProbeTec ET System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

3. Reproducibility Problems with the Abbott Laboratories LCx Assay for Chlamydia trachomatis and Neisseria gonorrhoeae

4. Johnson, R. E., W. J. Newhall, J. R. Papp, J. S. Knapp, C. M. Black, T. L. Gift, R. Steece, L. E. Markowitz, O. J. Devine, C. M. Walsh, S. Wang, D. C. Gunter, K. L. Irwin, S. DeLisle, and S. M. Berman. 2002. Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections—2002. Morb. Mortal. Wkly. Rep. Recomm. Rep.51(RR-15):1-38;CE1-CE4.

5. Reproducibility problems with the AMPLICOR PCR Chlamydia trachomatis test

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