Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, Including Confirmation with N. gonorrhoeae- Specific 16S rRNA PCR, with Traditional Culture-Reference-Cited by-同舟云学术

Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, Including Confirmation with N. gonorrhoeae- Specific 16S rRNA PCR, with Traditional Culture

Published:2005-03 Issue:3 Volume:43 Page:1445-1447
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Luijt Dirk S.¹,Bos Petra A. J.¹,van Zwet Anton A.¹,van Voorst Vader Pieter C.²,Schirm Jurjen¹

Affiliation:

1. Regional Public Health Laboratory

2. Department of Dermatology, University Hospital Groningen, Groningen, The Netherlands

Abstract

ABSTRACT A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae -specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.3.1445-1447.2005

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