Author:
Donaldson G C,Ball L A,Axelrood P E,Glass N L
Abstract
We examined the usefulness of primer sets designed to amplify introns within conserved genes in filamentous ascomycetes to differentiate 35 isolates representing six different species of Fusarium commonly found in association with conifer seedlings. We analyzed restriction fragment length polymorphisms (RFLP) in five amplified PCR products from each Fusarium isolate. The primers used in this study were constructed on the basis of sequence information from the H3, H4, and (beta)-tubulin genes in Neurospora crassa. Primers previously developed for the intergenic transcribed spacer region of the ribosomal DNA were also used. The degree of interspecific polymorphism observed in the PCR products from the six Fusarium species allowed differentiation by a limited number of amplifications and restriction endonuclease digestions. The level of intraspecific RFLP variation in the five PCR products was low in both Fusarium proliferatum and F. avenaceum but was high in a population sample of F. oxysporum isolates. Clustering of the 35 isolates by statistical analyses gave similar dendrograms for H3, H4, and (beta)-tubulin RFLP analysis, but a dendrogram produced by intergenic transcribed spacer analysis varied in the placement of some F. oxysporum isolates.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference54 articles.
1. Molecular phylogeny of northern hemisphere species of Armillaria;Anderson J. B.;Mycologia,1992
2. .Axelrood P. E. Unpublished data.
3. Seedborne Fusarium on Douglas-fir: pathogenicity and seed stratification method to decrease Fusarium contamination;Axelrood P. E.;New For.,1995
4. Bloomberg W. J. 1981. Disease caused by Fusarium in forest nurseries p. 178-187. In P. E. Nelson T. A. Toussoun and R. J. Cook (ed.) Fusarium: diseases biology and taxonomy. The Pennsylvania State University Press University Park.
5. An evaluation of Fusarium oxysporum from crucifers based on pathogenicity, vegetative compatibility and geographic origin;Bosland P. W.;Can. J. Bot.,1987
Cited by
69 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献