Affiliation:
1. Spice and Beverage Research Institute Chinese Academy of Tropical Agricultural Sciences Wanning China
2. National Center of Important Tropical Crops Engineering and Technology Research Wanning China
3. College of Plant Protection Henan Agricultural University Zhengzhou China
4. College of Mathematics and Physics Henan University of Urban Construction Pingdingshan China
5. School of Life Sciences Zhengzhou University Zhengzhou China
6. College of Tropical Crop Science Yunnan Agricultural University Puer China
Abstract
AbstractFusarium wilt has occurred in the main Piper nigrum cultivation regions, which seriously affects the yield and quality of P. nigrum. To identify the pathogen of this disease, the diseased roots were collected from a demonstration base in Hainan Province. The pathogen was obtained by tissue isolation method and confirmed by pathogenicity test. Based on the morphological observation, sequence analyses of TEF1‐α nuclear gene, Fusarium solani was identified as the pathogen causing P. nigrum Fusarium wilt and induced symptoms on inoculated plants, including chlorosis, necrotic spots, wilt, drying, and root rot. The experiments for the antifungal activity showed that all the 11 fungicides selected in this study showed certain inhibitory effects on the colony growth of F. solani, where 2% kasugamycin AS, 45% prochloraz EW, 25 g·L−1 fludioxonil SC and 430 g·L−1 tebuconazole SC exhibited relative higher inhibitory effects with EC50 as 0.065, 0.205, 0.395, and 0.483 mg·L−1, respectively, and were selected to perform SEM analysis and test in seeds in vitro. The SEM analysis showed that kasugamycin, prochloraz, fludioxonil, and tebuconazole might have exerted their antifungal effect by damaging F. solani mycelia or microconidia. These preparations were applied as a seed coating of P. nigrum Reyin‐1. The kasugamycin treatment was most effective in reducing the harmful impact of F. solani on the seed germination. These results presented herein provide useful guidance for the effective control of P. nigrum Fusarium wilt.
Funder
National Natural Science Foundation of China
Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences
Subject
Applied Microbiology and Biotechnology,General Medicine
Cited by
1 articles.
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