Affiliation:
1. Department of Microbiology, Technical University of Munich, Federal Republic of Germany.
Abstract
Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizations. The probes can be used for the specific detection and identification of colonies of the corresponding species in mixed cultures.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference13 articles.
1. A rapid procedure for colony screening using nylon filters;Buluwela L.;Nucleic Acids Res.,1989
2. Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization;Datta A. R.;Appl. Environ. Microbiol.,1987
3. A membrane filter technique for the detection of complementary DNA;Denhardt D. T.;Biochem. Biophys. Res. Commun.,1966
4. Reverse transcriptase sequencing of 16S ribosomal RNA from Faenia rectivirgula, Pseudonocardia thermophila and Saccharopolyspora hirsuta, three wall type IV actinomycetes which lack mycolic acids;Embley T. M.;J. Gen. Microbiol.,1988
5. DNA hybridization probe for the Pseudomonas fluorescens group;Festl H.;Appl. Environ. Microbiol.,1986
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