Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization-Reference-Cited by-同舟云学术

Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization

Published:1987-09 Issue:9 Volume:53 Page:2256-2259
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Datta A R¹,Wentz B A¹,Hill W E¹

Affiliation:

1. Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

Abstract

A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.53.9.2256-2259.1987

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