Boosting Secretion of Extracellular Protein by Escherichia coli via Cell Wall Perturbation

Abstract

Cell surface structure stabilization is important for extracellular secretion of proteins in Escherichia coli . As the main constituent of the cell wall, peptidoglycan contributes to cell structure robustness and stability. Here, we perturbed the peptidoglycan network by deleting dacA and dacB genes encoding d , d -carboxypeptidase enzymes to improve extracellular protein secretion. This new strategy could enhance the capacity of E. coli as a microbial cell factory for extracellular secretion of proteins and chemicals.