Secretion of streptavidin from Bacillus subtilis-Reference-Cited by-同舟云学术

Secretion of streptavidin from Bacillus subtilis

Published:1993-11 Issue:11 Volume:59 Page:3894-3898
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nagarajan V¹,Ramaley R¹,Albertson H¹,Chen M¹

Affiliation:

1. Central Research and Development Division, E. I. duPont de Nemours Company, Wilmington, Delaware 19880-0328.

Abstract

Streptavidin is an extracellular tetrameric protein produced by Streptomyces avidinii. A series of hybrid gene fusions consisting of Bacillus signal peptide coding regions fused to the mature streptavidin sequence were constructed. B. subtilis strains harboring these plasmids accumulate a tetrameric streptavidin in the growth medium. The properties of the streptavidin produced by B. subtilis are similar to those of the streptavidin produced by S. avidinii. B. subtilis strains carrying the various fusions can be grown to a high cell density in a biotin-free medium. Thus, B. subtilis represents an alternate host system for the production of streptavidin.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.59.11.3894-3898.1993

Reference16 articles.

1. Molecular cloning and nucleotide sequence of streptavidin gene;Argarana C. E.;Nucleic Acids Res.,1986

2. Isolation and properties of streptavidin;Bayer E. A.;Methods Enzymol.,1990

3. Single-step purification of avidin from egg white by affinity chromatography on biocytin-Sepharose columns;Cuatrecasas P.;Biochem. Biophys. Res. Commun.,1968

4. The purification of avidin and its derivatives on 2-iminobiotin-6-aminohexyl-Sepharose 4B;Heney G.;Anal. Biochem.,1981

5. Hofmann K. S. W. Wood C. C. Brinton J. A. Montibeller and APPL. ENvIRON. MICROBIOL.

Cited by 32 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Establishment of a method to detect human immunoglobulin G1 binding microorganisms;Diagnostic Microbiology and Infectious Disease;2023-11

2. Efficient secretory production of large‐size heterologous enzymes in Bacillus subtilis : A secretory partner and directed evolution;Biotechnology and Bioengineering;2020-07-09

3. Mini review: Recombinant production of tailored bio-pharmaceuticals in different Bacillus strains and future perspectives;European Journal of Pharmaceutics and Biopharmaceutics;2018-05

4. GAP promoter-based fed-batch production of highly bioactive core streptavidin byPichia pastoris;Biotechnology Progress;2016-05-04

5. Expression and purification of soluble monomeric streptavidin in Escherichia coli;Applied Microbiology and Biotechnology;2014-04-02