Horizontal Transfer of parC and gyrA in Fluoroquinolone-Resistant Clinical Isolates of Streptococcus pneumoniae-Reference-Cited by-同舟云学术

Horizontal Transfer of parC and gyrA in Fluoroquinolone-Resistant Clinical Isolates of Streptococcus pneumoniae

Published:2000-04 Issue:4 Volume:44 Page:840-847
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Ferrándiz María José¹,Fenoll Asunción²,Liñares Josefina³,De La Campa Adela G.¹

Affiliation:

1. Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Cientı́ficas), Centro Nacional de Biologı́a Fundamental,1 and

2. Servicio de Bacteriologı́a, Centro Nacional de Microbiologı́a,2 Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, and

3. Servicio de Microbiologı́a, Hospital Princeps d'Espanya, Ciutat Sanitària i Universitaria de Bellvitge, 08907 l'Hospitalet de Llobregat, Barcelona,3 Spain

Abstract

ABSTRACT We have analyzed genetically three clinical isolates (3180, 3870, and 1244) of Streptococcus pneumoniae with high-level ciprofloxacin resistance. Isolates 3180 and 3870 were atypical because of their insolubility in deoxycholate. However, they hybridized specifically with pneumococcal autolysin and pneumolysin gene probes and have typical pneumococcal atpC and atpA gene sequences. Analysis of the complete sequences of the parC and gyrA genes revealed total variations of 8 and 8.7% (isolate 3180) and 7.4 and 3.6% (isolate 3870), respectively, compared to the wild-type strain R6 sequence. The variations observed between the sequences of R6 and isolate 1244 were less than 0.9%. The structure of the gyrA and parC genes from isolates 3180 and 3870 was organized in sequence blocks that show different levels of divergence, suggesting a pattern of recombination. These results are evidence for recombination at the fluoroquinolone target genes in clinical isolates of S. pneumoniae . The genetically related viridans group streptococci could act as a reservoir for fluoroquinolone resistance genes.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.44.4.840-847.2000

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